Granulocyte Marker 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in IF, FACS, IHC (f), IHC (p) 和 ISt. Suitable for 人.
Quick Overview for Granulocyte Marker 抗体 (ABIN6163558)
抗原
Granulocyte Marker
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunostaining (ISt)
克隆位点
BM-1
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原理
- Mouse Monoclonal anti-Granulocyte Marker (BM-1), Purified, with BSA
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产品特性
- This antibody recognizes a 183 kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This MAb reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Human peripheral blood mononuclear cells
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亚型
- IgG1
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anti-Granulocyte Marker antibody
适用: 人 IF, FACS, IHC (f), IHC (p), ISt 宿主: 小鼠 Monoclonal BM-1 unconjugated
anti-Granulocyte Marker antibody适用: 人, Macaque IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal BM-2 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- No special pretreatment is required for staining of formalin/paraffin tissues
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HL60 cells. Bone marrow, lymph node or tonsil
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 200 μg/mL
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缓冲液
- PBS/0.05 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Granulocyte Marker
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背景
- Myeloid specific protein, p183 DNA-binding cytoplasmic protein
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分子量
- 183 kDa
抗原
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